To evaluate the assay's diagnostic performance, we tested a series of 91 serum samples (20 NMO patients, 41 MS patients and 30 HC), positive and negative controls provided by the manufacturer, and a commercial antibody to human AQP4 to define AQP4-Ab specific and non-specific staining patterns on the various substrates included in the biochip. Here, AQP4 is linked to neuromyelitis optica.