To confirm the cytoplasmic localization of Kaiso in CML-BP, we analyzed cytoplasmic expression of Kaiso protein by western blot analysis, comparing expression in cytoplasmic and nuclear protein extracts in K562 cell line (Figure1D) and imatinib-resistant K562 cell line (Figure1D). Here, ZBTB33 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.