Bisulfite genomic cloning and direct sequencing also showed that methylation involving both CpG sites within the proximal and the distal region of the 5′ UTR CpG island of the p14ARF flanking exon 1β (nucleotide position −69 to position +4 relative to the translation codon ATG) is not a frequent event in CRC, but seems to occur more particularly in tumors displaying a restricted pattern of p53 overexpression, including MSI-H and MSS tumors (Figure 5). This evidence concerns the gene TP53 and colorectal carcinoma.