Using bisulfite genomic cloning and direct sequencing, we found that extensive methylation involving both the proximal and the distal CpG sites within the 5′ CpG island of p14ARF flanking exon 1β was rare in CRC generally, but occurred more frequently in CRCs displaying a restricted pattern of p53 overexpression (Table 3). The gene discussed is TP53; the disease is colorectal carcinoma.