In summary, we applied the gene identification by nonsense-mediated mRNA decay inhibition (GINI) strategy to lymphoblastoid cell lines established from the blood of affected and unaffected members of three multiple-case non-BRCA1/2 breast cancer families but we did not identify any nonsense mutations that may underlie the breast cancer risk in any of the three families investigated. This evidence concerns the gene BRCA1 and breast carcinoma.