Indeed, we show here that EGF-induced Arf6 activation could be suppressed by ectopic expression with GEP100 siRNA as well as GEP100-△PH, so we suggest that the PH domain of GEP100 is involved in EGF signaling to induce Arf6 activation and migration of human hepatoma HepG2 cells. This evidence concerns the gene EGF and hepatocellular carcinoma.