Finally, we have not assessed if the tumor infiltrating CD8+ and Foxp3+ cells are targeted against specific tumor associated antigens or assayed for functional CD8+ using e.g. granzyme B. Furthermore, since Foxp3, although at low levels, may be transiently induced in CD4+ and CD8+ T-cells upon stimulation, all Foxp3+ TILs may not be Tregs [35]. This evidence concerns the gene CD4 and neoplasm.