For example, transcriptional repression of DACT3, which is an epigenetic regulator of Wnt/β-catenin signaling in colorectal cancer, was not associated with CpG promoter methylation, but the presence of the bivalent histone modifications H3K4me3 and H3K27me3 in colon cancer cells [66]. This evidence concerns the gene DACT3 and colorectal cancer.