Second, Garvet et al. subfractionated on discontinuous sucrose density gradients to equilibrium muscle membranes obtained under basal conditions and found a significant enrichment of GLUT4 in denser membrane fractions of type 2 diabetics compared with insulin-sensitive controls without any statistically significant differences in the recovery of membrane markers in any of the subfractions [56]. This evidence concerns the gene SLC2A4 and type 2 diabetes mellitus.