In the present study, we implemented the methylation signature of the 12 breast cancer candidate genes (APC, BIN1, BMP6, BRCA1, CST6, ESR-b, GSTP1, P14, P16, P21, PTEN and TIMP3) by comparing lymph nodes metastasis to their matched primary tumor tissues and normal tissues from the same breast cancer patients. This evidence concerns the gene TIMP3 and breast cancer.