Zbed4-SAFB1 close association was first identified by immunoprecipitation with Zbed4 antibody from Y79 retinoblastoma cell extracts and mass spectrometry of the separated proteins and was confirmed in vitro with a Zbed4 membrane overlay assay and in vivo by the presence of both proteins in the Zbed4 and SAFB1 co-immunoprecipitates from the same cell extracts. Here, SAFB is linked to retinoblastoma.