In 63 HCC sera tested with both anti-GRP78 autoantibody and AFP, 37 (58.7%) had an AFP level >100 ng/ml, 31 (49.2%) had an AFP level >200 ng/ml and 25 (39.7%) were positive with anti-GRP78 autoantibody. The gene discussed is HSPA5; the disease is hepatocellular carcinoma.