SIRT1 and Increased total leukocyte count: To understand if mIGF-1 cardiac transgene induced leukocytosis was dependent on SIRT1 activity, we analyzed the blood of mIGF-1 Tg where SIRT1 activity was specifically ablated in a conditional (αMHC-Cre) and tamoxifen-inducible manner in cardiomyocytes (mIGF-1 Tg; SIRT1 CKO), as we have recently described [6].