Studies were done by using one or two more markers like des-γ carboxyprothrombin (DCP) also called prothrombin induced by vitamin K absence II (PIVKAII) and glycosylated AFP-L3 (Lens culinaris Agglutinin-Reactive AFP) fraction serum levels to diagnose earlier HCC and increasing the sensitivity especially when HCC is associate with cirrhosis, HCV or HBV infection [71, 74, 77–81]. The gene discussed is AFP; the disease is hepatocellular carcinoma.