In most publications that tested OSNA for the detection of lymph node micrometastases and macrometastases, the cutoff was set at 250 CK19 mRNA copies/μL.13, 17, 19, 20 Although some of those investigations were performed in patients with breast cancer, the results of the molecular analyses are transferable to the setting of patients with colon cancer. The gene discussed is KRT19; the disease is breast cancer.