Although the change was most pronounced in PDK4, also PGC-1α (Figure 5B) and ERRα (Figure 5C) proteins showed a similar trend: both running and high-fat feeding increased the expression of each protein, but high-fat feeding combined with running had no additive effect on the protein expressions (no difference between the HF groups). The gene discussed is PPARGC1A; the disease is hydrops fetalis.