After 15 days of secondary transplantation, we analyzed the GFP+LSK and LSK− populations in bone marrow of the secondary CML mice, and detected by FACS both BCR-ABL+LSK and LSK− cells, demonstrating that the LSK to LSK− transition also occurs for LSCs (Fig. 4A). The gene discussed is ABL1; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.