Because only BCR-ABL+LSK cells are capable of self-renewing and initiating leukemia in secondary recipient mice, transferring LSC-containing bone marrow cells in CML mice to secondary recipient mice allows examination of the transition of BCR-ABL+LSK to BCR-ABL+LSK− cells. The gene discussed is ABL1; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.