To investigate the inductive properties of chicken CYP2D49 in vitro, Leghorn male hepatocellular carcinoma epithelial (LMH) cells were treated with four representative CYP inducers (rifampicin, clotrimazole, β-naphthoflavone and dexamethasone) and a candidate substrate of CYP2D49 (bufuralol). The gene discussed is PPIG; the disease is hepatocellular carcinoma.