ICC and western blot analysis showed a low level of the mescenhymal/fibroblast cell marker Thy-1 in the FbCs compared to the hLFbs (Figure S3 J-L), while a fibroblast-specific antigen, S100A4, was much higher in the FbCs (Figure S3 G-I). This evidence concerns the gene S100A4 and intrahepatic cholangiocarcinoma.