CD8A and neoplasm: In contrast, we observed a significant decrease of CD69+/CD25+ CD8+ T cells in tumor-draining lymph nodes (tdLN) of high tumor-burdened LLA-TG-3 mice compared to low tumor-burdened LLA-TG-3 mice in PMA-supplemented medium (p < 0.005; Mann–Whitney U test; Fig. 4b, c).