In detail, CD69+ CD4+ cells were more frequent in the high tumor burden compared to the no tumor group (p < 0.05; Fig. 2b); CD25+ CD8+ cells were more frequent in high tumor-burdened animals compared to tumor-free LLA-TG-3 mice (p < 0.05; Fig. 2f), and CD69+/CD25+ CD4+ cells were more frequent in the low tumor burden compared to the no tumor group (p < 0.005; all Dunn’s multiple comparison post hoc test; Fig. 2d). This evidence concerns the gene CD8A and neoplasm.