In contrast, however, a significantly increased frequency of CD4+CD25+FoxP3+ T cells was present in lymph nodes of tumor-burdened animals compared to lymph nodes of tumor-free LLA-TG-3 mice (p < 0.0001 for both the low and high tumor burden compared to the no tumor group; one-way ANOVA with Dunnett’s multiple comparison post hoc test; Fig. 5a, b). This evidence concerns the gene CD4 and neoplasm.