However, since increased Treg numbers are likely not the sole immunosuppressive event during melanoma progression in these mice, we addressed IL-10 and TGF-β1 levels in tumor-draining LN and tumors of LLA-TG-3 mice, particularly, as both cytokines have been associated with both melanoma and Treg development. The gene discussed is TGFB1; the disease is melanoma.