The average integral optical density (IOD) of positive signal representing the relative neogenin level was significantly different between grade I and III, grade I and IV, grade II and III, and grade II and IV (One-Way ANOVA, n = 69, p<0.01, Fig. 2E), and also was distinct between the low-grade and the high-grade gliomas (independent t-test, n = 69, p<0.05, Fig. 2F, Table 2). The gene discussed is NEO1; the disease is glioma.