Human placental cDNA, where the expression of ARF6 and CYTH3 mRNAs has been previously reported, MDA-MB-231 breast cancer cells (which known to express ARF1, ARF6 and CYTH1-4) cDNA and plasmid carrying ARF1, ARF6, CYTH1, CYTH2, CYTH3 or CYTH4 cDNA were used as positive controls along with water and –RT cDNA as negative controls in the RT-PCR analysis [23], [26], [27], [28]. Here, ARF1 is linked to breast cancer.