Pre-treatment of PBMCs with anti-CD16 antibodies remarkably reduced the ADCC reaction mediated by ALS-IgG, while an 18 h incubation of ALS-IgG with CD16-transfected BW cells or peripheral NK cells induced IL-2 or IFNγ, respectively. The gene discussed is IFNG; the disease is amyotrophic lateral sclerosis.