In the wt form of the four model NPs, the frequency of M conformers ranged from 0% in Q19 (Q21 is the minimum pathological threshold detected in polyQ diseases, specifically in SCA6 [2]) to 64% in Sup35NM, which for each NP was positively correlated with amyloid fiber density (Figures 2–, , 5, S2D, S3D, S4D, S5D, S6D; Table S1). This evidence concerns the gene CACNA1A and amyloidosis.