Subjects II.3 and III.3 did not display hypoalphalipoproteinemia and impaired cholesterol esterification in plasma except for a modest reduction of cholesterol esterification rate; this finding is in contrast with the average profile of Italian heterozygous carriers of LCAT mutations, which have unesterified/total cholesterol ratios greater than 0.30 and reduced LCAT activity.3 Here, LCAT is linked to hypoalphalipoproteinemia.