To more thoroughly study the function of LEF-1 in colon cancer, we constructed three colon cell lines that stably expressed the different LEF-1 phenotypes: SW480 expressing truncated LEF-1 (SW480-LEF-1-ΔL), HT29 expressing truncated LEF-1 (HT29- LEF-1- ΔL) and HT29 expressing the full-length LEF-1 (HT29-LEF-1-FL). The gene discussed is LEF1; the disease is malignant colon neoplasm.