These data are in line with highly reducedproliferation of various cancer cell lines resulted from the down-regulation of of PI3K/Akt signaling [1, 4]Importantly, unlike PD98059, prolonged treatment with LY294002 (up to 48 hours) induced significant apoptosis in A431 cells as was evident by FACS analysis (Figure 5C). The gene discussed is AKT1; the disease is cancer.