The SRF50 gene list and the Top 50 ANOVA gene list achieved the second highest subtype prediction accuracy of the underrepresented subtype (HER2, N = 11, 63.6%) in contrast to 54.5% using the Parker et al. gene list and 36.4% from the overall ANOVA gene list(Table 3 BC, Additional file 1: Table S 10 & Additional file 1: Figure S 7). This evidence concerns the gene ERBB2 and breast cancer.