TF and brain glioma: In this study, we aim to determine: firstly, the development and characterization of Tf-SPIONs as well as whether it is suitable for MR imaging; secondly, whether Tf-SPIONs could be specifically and efficiently internalized by C6 glioma cells and detected by MR imaging; and thirdly, whether Tf-SPIONs could act as a specific targeting MRI contrast agent for brain glioma in vivo without saturation of the TfRs on BBB.