ENAH and neoplasm: When this experiment was conducted in “sensitized” flies, which have enlarged eyes, but no tumor formation, knock-down of Ena induced de novo tumor formation in 17% of eyes and metastasis formation in 4% of flies, compared to negative control (white gene) which did not result in any tumor or metastasis formation (Fig. 4E, 4G, 4J–K and 4N–O).