To delineate how methylation at various regions flanking the RARRES1 promoter interact with each other and with cofactors for exerting ultimate silencing effect, we began to evaluate the methylation occurring at sequences between −664 and +420 (reference to TSS set as +1) in 18 pairs of primary breast tumors as well as their matched benign tissues. This evidence concerns the gene RARRES1 and breast neoplasm.