In the current study, we aimed to gain more insight into the possibility of treating chondrosarcoma patients with hormonal therapy by further investigating the expression of the hormone receptors ESR1 and AR, and of aromatase, the enzyme that mediates the last step in the biochemical formation of estrogen, in a larger set of conventional chondrosarcomas as well as three rare chondrosarcoma subtypes. Here, ESR1 is linked to chondrosarcoma.