ANXA5 and systemic lupus erythematosus: To determine whether SLE and diosgenin can induce cell apoptosis, WEHI-3 cells treated with 200 μg/mL of SLE or 50 μM of diosgenin in WEHI-3 cells for 12 h triggered the translocation of phosphatidylserine (PS) from inner side of the plasma membrane to the outer layer of the cell membrane which was examined by Annexin V/PI analysis (Annexin positive cells: 32.89 ± 2.26% and 41.93 ± 3.50%) as shown in Figure 3(a).