GPC3 and hepatocellular carcinoma: Nevertheless, a previous study using the same protocol of GPC3 immunohistochemical staining has shown that GPC3-positive HCC patients (>10% of GPC-positive cells in immunohistochemical staining) have a lower 5-year survival rate than GPC-negative HCC patients (<10% of GPC-positive cells in immunohistochemical staining [46].