We then further focused on validating the modulation of PIWIL1 gene expression in lung cancer cells over-expressing RASSF1C using immunostaining and Western blot analyses (Figures 3 and 4), we also found that silencing of RASSF1C resulted in reduction of PIWIL1 expression (Table 2 and Figure 5). This evidence concerns the gene PIWIL1 and lung carcinoma.