A second generation 3PO derivative (PFK015) was recently reported to more potently inhibit recombinant human PFKFB3 activity, F2,6BP synthesis and proliferation in cancer cells and to display markedly improved pharmacokinetic properties (2011 American Association for Cancer Research Annual Meeting, Abstract #2825) and a related compound is proceeding into phase I clinical testing in cancer patients. This evidence concerns the gene PFKFB3 and cancer.