A more detailed comparative analysis of KPL-4 cells taken from cell culture and circulating KPL-4 cells in blood from tumour-bearing mice revealed changes in expression of EMT-associated genes with upregulation of Twist, Collagen-alpha-2(V)-chain (COL5A2), epidermal growth factor receptor (EGFR), moesin (MSN) and beta-type platelet-derived growth factor receptor (PDGFRB) in the latter. The gene discussed is TWIST1; the disease is neoplasm.