To determine when these two events occurred and to assess if their disappearance was correlated to their accumulation in the inclusion, we established the expression and distribution profile of ACBD6 and ZNF23 in infected cells during the developmental cycle of Ct. At the mRNA level, we could not detect a significant change in expression of ABCD6 and ZNF23 from 0 to 36 hr post-infection for Ct strains D and L2 (Figure 8). The gene discussed is ACBD6; the disease is infection.