Based on the quantitative RNA analysis from the different groups, ischemia-reperfusion injury (IR and IR+GF) induced a stronger up-regulation of c-Kit, Isl1 and Nkx2.5 versus the control group than both myocardial infarction and pregnancy (Fig 2A, 3A, 4A). The gene discussed is NKX2-5; the disease is ischemia reperfusion injury.