In order to further characterize the E2-specific responses in patients clearing usual VIN after treatment, we analyzed IFNγ and IL2 production by HPV16 E2-stimulated CD4+ T-cells from women whose peripheral blood mononuclear cells (PBMC) displayed positive proliferative responses (F#2, #3, #4, #5, #6 and #8). This evidence concerns the gene IFNG and vulvar intraepithelial neoplasia.