We further did real-time RT-PCR analysis of 5 genes known to be involved in tumor development and progression: secreted protein, acidic, cysteine-rich (osteonectin, Sparc), leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1), tumor protein D52 (TPD52), egl nine homolog 2 (EglN2) and cyclin D1 (CCND1) using RNA from cells used in the microarray analysis. This evidence concerns the gene SPARC and neoplasm.