While NPC cells seemed to be insensitive to IL-1α induction of NF-κB signaling, positive controls showed that TNF-α induced degradation of inhibitor kappa B (IκBα) protein (Figure S1A) and subsequent nuclear translocation of a NF-κB subunit p65 (Figure S1B), indicating activation of NF-κB signaling [36] and confirming the functional integrity of TNF-α in this experimental setting. The gene discussed is TNF; the disease is nasopharyngeal carcinoma.