AGT and cardiac hypertrophy: To determine whether overexpression of KLF15 represses pressure overload-induced cardiac hypertrophy, we induced hypertrophy a week after injecting mice with the rAAV9-KLF15 and rAAV9–GFP virus, through continuous infusion of AngII (1.5 μg/g/day) via subcutaneously placed osmotic minipumps (Figure 3b) [16].