NEUROD1 and Hyperglycemia: Moreover, it was reported that mild hyperglycaemia can affect beta-cell phenotype [37] with a progressive loss of beta-cell differentiation markers (Pdx-1, BETA2/NeuroD, NKX6.1, and HNF-1β) and genes involved in glucose sensing (GLUT-2 and glucokinase) [37, 42].