To verify that the IFNβ-luciferase reporter induction observed in the primary and secondary screens correlates with induction of IFNβ mRNA and associated IFN-stimulated genes (ISG) in human cells, we infected human lung carcinoma (A549) cells with influenza A/WSN/33 virus, treated them with either ASN2 or DMSO, and examined expression of IFN-related genes by qRT-PCR. Here, IFNB1 is linked to lung carcinoma.