CD14 and infection: However, as Hla-dependent plasma membrane permeability to T cells and B cells required at least 60 minutes to become apparent and mechanisms that did not require Hla caused substantial plasma membrane permeability to the majority CD14+ PBMCs within 30 minutes, we further examined CD14+ PBMC plasma membrane permeability following intoxication with diluted USA300, USA300Δhla, USA300Δhla comp, and USA300ΔsaeR/S supernatants (Figure 4D) as well as during infection of PBMCs with these strains (Figure 4E).