To determine if Hla-dependent plasma membrane permeability is the result of events associated with programmed cell death, we performed Annexin V binding and ApoBrdU TUNEL assays on PMNs and PBMCs following infection with USA300, USA300Δhla, USA300Δhla comp, or USA300ΔsaeR/S (Figure 6). Here, ANXA5 is linked to infection.