PBMCs infected with USA300, USA300Δhla, USA300Δhla comp, or USA300ΔsaeR/S for 6 hours then stained with PI and either anti-CD4 or anti-CD8 (Figure 4C) also demonstrated that Hla significantly contributes to T cell plasma membrane permeability during infection of PBMCs with USA300. The gene discussed is CD8A; the disease is infection.