TP53 and infection: Figure 2 shows that in uninfected cells, p53 expression is low and appears evenly distributed throughout the cytoplasm with only basal levels seen in the nucleus. Upon infection with MP-12 (visualized with the RVFV N protein), p53 expression is increased overall and p53 localizes within the nucleus with slight distribution within the cytoplasm. Interestingly, the nuclear p53 correlated with RVFV infection, as detected by RVFV N. To confirm these results, nuclear and cytoplasmic extracts were analyzed for the expression of p53 in mock or MP-12 infected cells (Figure 2B).