We then infected HuT/CCR5 cells with HIV-1 Vpr+/Ampho and HIV-1 Vpr−/Ampho stocks at an MOI of 1 and assessed luciferase expression at 3 dpi, since peak infection was reached at this time-point with the VSV-G pseudotyped virus infection (Fig. 3A). This evidence concerns the gene CCR5 and viral infectious disease.