To further our understanding of the role of NKCC1 in GB cell migration and invasion, we investigated (1) whether the expression of NKCC1 in human tumors correlates with tumor grade, (2) whether NKCC1 affects cell contractility and migration, (3) whether NKCC1 can have an effect on the interaction between the cells and the cells' adhesion substratum, and (4) whether a signaling mechanism involved in the regulation of NKCC1 by promigratory factors exists in GB cells. This evidence concerns the gene SLC12A2 and neoplasm.