Comparable IL-24-induced anti-tumor activity in vitro was observed in thethree HCC cell lines which differed by their metastatic potential; these datafurther supported the findings that tumors which are sensitive to mitochondrialdysfunction and apoptosis induced by SG600-IL-24 have a range of defects in variousproteins including p53, p16/INK4a, and Rb [4,30,31]. This evidence concerns the gene RB1 and neoplasm.