Although blockade of HMGB1 in an APAP-induced ALI model does not protect against liver injury at 9 h point, inflammation is reduced as seen by myeloperoxidase (MPO) activity in total liver extract [9], however, the later time points are not studied and the role of HMGB1 in APAP overdose is still not known. The gene discussed is HMGB1; the disease is acute respiratory distress syndrome.